BD FACSCanto II Flow Cytometer

BD FACSCanto II Flow Cytometer

The BD FACSCanto II from BD Biosciences is an advanced, compact flow cytometer designed for multiparametric cell analysis. The system is intended for clinical laboratories, research institutions, and biomedical applications where accurate, reproducible, and reliable analytical results are essential.

The FACSCanto II utilizes a two- or three-laser configuration and is capable of detecting up to 8 fluorescent colors. This allows for the simultaneous analysis of multiple cellular characteristics, making the instrument particularly well-suited for immunophenotyping, cell characterization, and hematological research.

A key feature is its fixed optical alignment (Fixed Alignment Flow Cell), which eliminates the need for manual laser alignment by the user. This reduces start-up time and contributes to consistent, reproducible measurement results.

The advanced optical system optimizes signal detection, providing high sensitivity and resolution for multicolor analyses. With an automated fluidics system, comprehensive quality control, and high processing speed, the BD FACSCanto II is well-suited for laboratories with high sample throughput.

Key Features

Flow cytometric analysis with up to 8 fluorescent colors.

2- or 3-laser configuration.

Three available laser wavelengths: 405, 488, and 633 nm.

Fixed optical alignment; no manual adjustment required.

8 fluorescence detectors (PMTs).

High sensitivity and resolution for multicolor analyses.

Sample processing rate of up to 10,000 events per second.

Automated fluidics system.

Suitable for clinical diagnostics and scientific research. Reproducible measurement results and comprehensive quality control. Technical specifications

1. Laser system

Specification Value

Manufacturer BD Biosciences

Model FACSCanto II

Device type Flow cytometer

Laser configuration 2 or 3 lasers

Violet laser 405 nm solid state diode

Violet laser power 30 mW (fiber output)

Blue laser 488 nm solid state

Blue laser power 20 mW

Red laser 633 nm HeNe

Red laser power 17 mW

Laser configuration Spatially separated laser beams

Laser spot size 9 × 65 µm (elliptical)

Optical alignment Fixed (Fixed Alignment)

2. Flow cell and optical system

Specification Value

Flow cell Rectangular quartz flow cell

Flow cell dimensions 180 × 430 µm

Collection optics Optical-gel coupled lens

Numerical aperture 1.2 NA

FSC resolution 1.0 µm

SSC resolution 0.5 µm

Fluorescence detection Reflective optics

Fluorescence detectors 8 PMTs

Detector configuration 4-2-2

3. Fluorescence detection

Laser Fluorochromes

Violet (405 nm) Pacific Blue, AmCyan

Blue (488 nm) FITC, PE, PerCP, PerCP-Cy5.5, PE-Cy7

Red (633 nm) APC, APC-Cy7

Detector bands:

Laser Detector Band

Violet 450/50nm

Violet 502–525 nm

Blue 530/30nm

Blue 585/42nm

Blue >670 nm

Blue 780/60nm

Red 660/20nm

Red 780/60nm

4. Detection and sample capacity

Specification Value

FSC detector Photodiode

SSC detector PMT

Fluorescence detectors 8 PMTs

FITC sensitivity <100 MESF

PE sensitivity <50 MESF

Maximum particle size 50 µm

Sample flow rate 10–120 µL/min

Maximum acquisition rate 10,000 events/sec

Fluorescence parameters 6 compensated parameters

Scatter parameters 2

Sample dead volume 30 µL

Compatible sample tubes BD Falcon 12 × 75 mm

5. Dimensions and weight

Cytometer – Fluidics cart

Depth 610mm – 610mm

Width 910mm – 790mm

Height 640mm – 640mm

Weight 145 kg – 51 kg

Total system configuration:

Cytometer: 145 kg

Fluidics cart: 51 kg

Total weight: 196 kg