Pall ForteBio BliTz Label-Free Protein Analysis System

The BLItz BLI system is a compact platform that measures biomolecular interactions in real time using biolayer interferometry (BLI). The system works by detecting changes in optical interference patterns that occur when molecules bind to a biosensor surface.

Unlike traditional methods that require fluorescent or radioactive labels, BLItz enables label-free analysis, preserving natural binding behavior. Thanks to the “dip-and-read” format, users can immerse disposable biosensors directly into microvolume samples, making the system highly accessible for routine protein studies. Key applications are:

Analysis of protein-protein interactions
Binding studies between antibodies and antigens
Kinetic and affinity measurements (association/dissociation rates)
Screening of binding specificity
Concentration and quality controls in purified samples

The system is generally regarded as an entry-level BLI platform, ideal for laboratories performing real-time kinetic analysis for the first time. Technical specifications (typical)

Measurement technology
Principle: Biolayer interferometry (detection based on optical interference)
Format: Dip-and-read biosensor technology
Detection type: Label-free optical measurement of biomolecular binding
Performance capabilities
Measured parameters:
Association rate (ka)
Dissociation rate (kd)
Equilibrium dissociation constant (kD)
Kinetic analysis: Real-time generation of binding curves
Affinity range: Suitable for interactions from low nM to high pM (depending on the assay design)
Sample requirements
Sample volume: Low-volume microplate-based assays (typically a few µL per test)
Throughput: Single-channel (entry-level model, low-throughput system)
Biosensors: Disposable, pre-coated, or functionalized tips