Multidetection microplate readers with dual-monochromators, dual-mode cuvette ports, and top and bottom reading capability. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command. The system uses two scanning monochromators to determine optimal excitation and emission settings. Changing methods or fluorophores requires only a few mouse selections to optimize the system.
Applications:
• DNA/RNA/protein quantitation and purity
• PicoGreen/NanoOrange/Bradford
• ELISAs/enzyme kinetics (i.e., Km, Ki, etc.)
• Drug dissolution profiles
• Live/Dead Viability/cytotoxicity assays
• Caspase-3 and protease assays
• cAMP assays using CatchPoint® Assay Kits
• Low volume applications
TECHNICAL SPECIFICATIONS:
Absorbance photometric performance:
Wavelength selection: Monochromator, tunable in 1 nm increments
Wavelength range: 200–1000 nm
Wavelength bandwidth: ≤ 4.0 nm
Wavelength accuracy: ±2.0 nm
Wavelength repeatability: ±0.2 nm
Photometric range: 0–4.0 OD
Photometric resolution: 0.001 OD
Photometric accuracy (microplate): < ±0.006 OD ±1.0%, 0–2 OD
Photometric accuracy (cuvette): < ±0.005 OD ±1.0%, 0–2 OD
Photometric precision: < ±0.003 OD ±1.0%, 0–2 OD
Stray light: < 0.05% @ 230 nm
Flourescence photometric performance:
Dual monochromators: 1 nm increment selection
EX Wavelength range 250–850 nm
EM Wavelength range: 250–850 nm
Wavelength bandwidth (EX, EM): 9 nm
Top-read optimized sensitivity: 5 pM (fluorescein) in 96 wells
Top-read guaranteed sensitivity: 15 pM (fluorescein) in 96 wells
Bottom-read optimized sensitivity: 10 pM (fluorescein) in 96 wells
Bottom-read guaranteed sensitivity: 25 pM (fluorescein) in 96 wells
General photometric performance:
Plate formats: 6, 12, 24, 48, 96, 384 wells
Light source: Xenon flash lamp (1 joule/flash)
Detector: Photomultiplier tube (PMT)
Read time-
96 wells: Abs 18 sec., FI 15 sec.
384 wells: Abs 49 sec., FI 45 sec.
Shaker time: 0 to 999 seconds
Temperature control: Ambient +4°C to 45°C
Temperature uniformity: < 1°C at 37°C set point
Temperature accuracy: ±1°C at 37°C set point
General specifications:
Dimensions (in.): 8.6 (H) x 22.8 (W) x 15 (D)
Dimensions (cm): 22 (H) x 58 (W) x 38 (D)
Weight: 35 lbs. (15.9 kg)
Power consumption: < 125 watts
Power source: 100–240 Vac, 3 A, 50/60 Hz
Robot compatible: Yes
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