Varian UV-VIS Spectrophotometer Cary 100

The Varian UV-VIS Spectrophotometer Cary 100 is a robust and reliable dual-beam instrument used for routine laboratory analysis and research. It is designed to determine the concentration of a substance by measuring the absorption of ultraviolet and visible light.

Key Features and Specifications

Optical Design: It is a dual-beam spectrophotometer with a dual chopper system, which ensures excellent photometric stability by placing the sample and reference images at the same point on the detector. The Cary 100 uses a single-dispersion monochromator (unlike the dual-dispersion Cary 300).

Wavelength Range: The instrument has a wide wavelength range from 190 to 900 nm.

Light Source: It uses both a tungsten-halogen lamp (for visible light) and a deuterium lamp (for UV light). Software: The spectrophotometer is controlled by the modular, Windows-based Cary WinUV software, which offers a user-friendly interface for various applications.

Photometric Range: It has a working range of over 4.0 absorbance units (Abs), often eliminating the need for sample dilution.

Variable Slit Widths: Variable slit widths provide optimal control over spectral resolution.

Robust Construction: The optics are sealed with a quartz coating to prevent exposure to corrosive environments and extend the instrument’s lifespan.

Accessories: A wide range of accessories is available, including temperature control, multi-cell holders, and diffuse and specular reflectance accessories, increasing flexibility in sample types and sizes.

Applications

The Varian Cary 100 is suitable for a variety of applications in routine and research environments, such as:

Concentration Determination: Measuring the concentration of dissolved substances using the Beer-Lambert law. Kinetic studies: Determination of reaction rates and enzyme activity.

DNA/RNA analysis: Determination of the purity and concentration of nucleic acids and measurements of thermal denaturation/renaturation of DNA.

Solid-state measurements: Analysis of films, filters, and textiles using special holders and reflection accessories.

Color measurements: Determination of color parameters using the diffuse reflection accessory and associated software.