Eppendorf BioSpectrometer Basic Compact UV/Vis Spectrophotometer

Eppendorf BioSpectrometer Basic

The Eppendorf BioSpectrometer Basic is a compact UV/Vis spectrophotometer designed for use in molecular biology, biochemistry, biotechnology, and cell biology. The instrument is suitable for quantitative analyses of DNA, RNA, and proteins, bacterial density measurements (OD600), color assays, and general UV/Vis absorbance measurements. The system operates standalone with built-in software and requires no external computer. Optical Properties

* Measuring Principle: Single-beam UV/Vis absorption spectrophotometer with reference path
* Wavelength Range: 200–830 nm
* Wavelength Step: 1 nm
* Spectral Bandwidth: ≤ 4 nm
* Wavelength Accuracy (Systematic): ± 1 nm
* Wavelength Reproducibility (Coincidental): ≤ 0.5 nm
* Light Source: Xenon Flash Lamp
* Detector: Photodiode Array / CMOS Sensor

Photometric Properties

* Absorption Range: 0–3.0 A
* Absorption Resolution: 0.001 A
* Photometric Accuracy (Systematic): ± 1% at 1.0 A
* Photometric Reproducibility (Coincidental): ≤ 0.002 A at 0 A and ≤ 0.005 A (0.5%) at 1 A A

Cuvettes and Sample Volumes

* Cuvette holder: 12.5 × 12.5 mm
* Suitable cuvettes: Glass, plastic, and UV-transparent cuvettes (such as quartz or UV plastic)
* Compatible with micro-volume cuvettes: Yes (e.g., for DNA/RNA measurements)
* Light path (optical path height): approx. 8.5 mm

Functionality

* Single-wavelength measurement
* Multi-wavelength measurements
* Spectrum scan (200–830 nm)
* Pre-programmed methods for:

* DNA and RNA quantification
* Purity ratios (e.g., A260/A280)
* Protein measurements (UV 280 nm or color assays)
* Bacterial density (OD600)
* Freely programmable methods
* Step-by-step user interface
* Storage of over 1000 results in internal memory

Use and Operation

* Large color display (TFT)
* Stand-alone operation via keyboard
* Export options via USB or printer (no PC required for operation)

Electrical Specifications

* Power supply: 100–240 V, 50–60 Hz
* Power: approx. 15 W during operation and approx. 5 W in energy-saving mode

Environmental Conditions

* Operating temperature: 15–35°C
* Relative humidity: 25–70%
* Operating pressure: 86–106 kPa
* Max. Altitude: up to 2000 m above sea level

Dimensions and Weight

* Dimensions (W x D x H): approx. 295 × 400 × 150 mm
* Weight: approx. 5.4 kg

Typical Applications:

* Quantification of DNA and RNA
* Analysis of nucleic acid purity (A260/A280, A260/A230)
* Protein concentrations (UV-280 or colorimetric assays)
* OD600 measurements for bacterial growth
* General UV/Vis measurements and spectrum scans