The FLUOstar OPTIMA is a versatile microplate reader offering four separate reading modes with integrated resonance energy transfer detection capabilities.
The FLUOstar OPTIMA reads up to 384-well plates in all detection modes and provides 1536-well plate capability for fluorescence intensity measurements.
On-board reagent injectors can be used to initiate kinetic events.
Due to the individually adjustable delivery volumes for each well, the injectors can be used to produce dilution schemes and concentration ranges throughout the plate. Top and bottom plate reading, precise temperature control up to 45°C, well scanning, multi-mode shaking capabilities and gas vent connection enhance the assay fl exibility of the FLUOstar OPTIMA.
Detection Modes: Fluorescence Intensity – including FRET, Luminescence (flash and glow) – including BRET, Time-Resolved Fluorescence – including DELFIA, UV/Vis absorbance
Measurement Modes: Top and bottom reading, Endpoint and Kinetic measurements, Sequential Multi Excitation measurements, Sequential Multi Emission measurements, Ratiometric measurements, Well Scanning
Microplate Formats: 6 to 384-well plates, user-definable
Light Source: High energy xenon flashlamp
Detector: Side window photomultiplier tube
Optical Filters: Excitation and emission filter wheels for 8 filters each
Spectral Range: 240 to 740 nm
Sensitivity: FI < 1 fmol / well Fluorescein, TRF < 70 amol/well Europium, LUM < 30 amol/well ATP – DLReady certified, ABS Dynamic range: ±0.000 – 4.000 OD – Reproducibility: ±0.010 OD for 0 – 2 OD range
Read Times: Flying mode: 15 s (96), 30 (384)
Reagent Injection: Up to 2 built-in reagent injectors, Injection at measurement position (6 to 384-well), Individual injection volumes for each well (3 to 350 µL), Variable injection speed up to 420 µL/s, Up to four injection events per well, Reagent back flushing
Shaking: Linear, orbital, and double-orbital with user-definable time and speed
Gas Vent: System to inject an atmosphere or to pull a vacuum into the reader
Incubation: +5°C above ambient up to 45°C
Dimensions (WxDxH): 440 x 480 x 260 mm
Weight: 26 kg
Included:
PC
Emission filter wheel filters: 405, 460, 420-P, 590, 540BP10 nm
Excitation filter wheel (excitation) filters: 355, 390, 485-P, 544 nm filters
Excitation filter wheel (absorbtion) filters: 405, 595, 492, 620 nm
Extra absorbtion filter: 450 nm
| Weight | 0,0 kg |
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