Thermo PikoReal 24 Real Time PCR System

The Thermo Scientific PikoReal 24 cycler is ideal for starting real-time PCR for low sample throughput (24-well format). The PikoReal software offers simple and intuitive cycler operation and allows easy online control from your computer. The cycler has 5 detection channels, which detect hydrolysis (5’Nuclease / TaqMan) probes either as single color or multiplex samples.

Thermal block

Block format: 24 well

Sample volume: 10 – 50 μl

Consumables: Low profile strip tubes and PCR tubes

Max heating rate: > 5°C/sec

Max cooling rate: 4.5°C/sec

Temperature range: 4-99.9°C

Temperature accuracy: +/-0.2°C

Temperature uniformity: ±0.3°C at 95°C, ±0.15°C at 60°C, ±0.2°C at 72°C

Heated Lid

Temperature range: 50-110°C

Control: Automatic temperature and pressing setting

Optics

Excitation: 5 LEDs

Excitation range: 475-640 nm

Detection: CCD

Detection range: 520 – 740 nm

Measuring time: all channels < 13 s, single channel < 3 s

Filter wavelength range:

Channel 1 – Excitation: 475 – 500 nm, Emission: 520 – 550 nm

Channel 2 – Excitation: 515 – 535 nm, Emission: 557 – 590 nm

Channel 3 – Excitation: 570 – 590 nm, Emission: 615 – 650 nm

Channel 4 – Excitation: 600 – 640 nm, Emission: 666 – 740 nm

Channel 5 – Excitation: 475 – 500 nm, Emission: 520 – 590 nm

Multiplex: Up to 4 targets

Dynamic range: 10 logs

Sensitivity: 1 copy

Analysis modes

Absolute quantification; Relative quantification; Melt curve

analysis; Allelic discrimination; High Resolution Melt (HRM)

Stand-alone or PC control:

The PikoReal qPCR system is controlled by PikoReal software, which allows remote monitoring of any qPCR experiment online from your own computer. Alternatively, you can save the qPCR protocol on a USB stick and load to the PikoReal instrument for stand-alone use.

PikoReal software include modules for absolute and relative quantification, melting curve analysis, allelic discrimination and High Resolution Melting (HRM). Advanced algorithms provide effortless data normalization without the need for an external reference dye.

Dimensions (WxDxH): 300 x 230 x 310 mm

Weight: 9.2 kg

Including software