Beckman Coulter Navios Flow Cytometer

Lasers:

Blue Solid State: 488 nm, 55 mW, Software Controlled

Red Solid State Diode: 638 nm, 50 mW, Software Controlled

Violet Solid State: 405 nm, 80 mW, Software Controlled

Beam-Shaping Optics: Cross cylindrical lenses 10 mm by 80 mm.

Blue Laser Beam Spot Size: An elliptical spot 10 μm high by 84 μm wide

Red Laser Beam Spot Size: An elliptical spot 9.6 μm high by 72 μm wide.

Violet Laser Beam Spot Size: An elliptical spot 8.9 μm high by 70 μm wide.

Laser Beam Separation: The laser beams are 125 μm (±12.5 μm) apart

Flow cell: BioSense 150 μm x 460 rectangular channel with an integral lens, mounted with avertical (upward) flow path.

Detector filters: See image

Sensors:

The FS sensor and the SS sensor are photodiodes.

The FL sensors are photo-multiplier tubes (PMTs) that have a 200-nm to 800-nm spectral range

Flow rates: Continuous pressure is applied to the sample tube based on user selected flow rates: Low (10 µL/min), Medium (30 µL/min) and High (60 µL/min)

Sheath Consumption: 780mL/h

Performance: Carryover: < 0.1%, Dead volume as low as 2 µL when using Beckman Coulter 12 x 75 mm polypropylene tube and adjustable probe

Acquisition modes: 32 tube Multi Carousel Loader (MCL), Single tube sampling mode, Automated work list acquisition, Manual work list mode

Throughput: 10000 normal leukocytes is 82 tubes/hour

Scatter resolution: Resolves 0.4 micron particles from background noise using Forward Scatter, Maximum detection of up to 40 microns

Fluorescence sensitivity: FITC < 107 MESF, PE < 64 MESF, PC5 < 13 MESF

Acquisition rate: 25000 events per second, 90% yield

Cytometer dimensions (WxHxD): 950 x 610 x 730 mm

Cytometer weight: 104 kg

Supply cart dimensions (WxHxD): 724 x 298 x 495 mm

Supply cart weight: 30 kg

PC and Software included.