Biorad NGC Quest 10 Plus Chromatography System

Bio-Rad NGC Quest 10 Plus

The Bio-Rad NGC Quest 10 Plus is an automated liquid chromatography system for protein, peptide and nucleic acid purification and method development. The system uses two independent system/gradient pumps (A and B), a multi-channel UV/Vis detector with simultaneous measurement of up to four wavelengths and a conductivity detector. The system is controlled with ChromLab Software, which allows chromatographic methods to be programmed, automated and analyzed.

The version described here has been expanded with a separate sample pump (up to 100 mL/min), inlet valve module and column switching valve module. This makes the system suitable for automated multi-dimensional chromatography (Multi-D), where, for example, multiple chromatographic columns can be used in succession or according to a programmed sequence. Bio-Rad specifically describes an NGC Quest 10 Plus Multi-D setup with sample pump, buffer inlet valves, column switching valves and outlet valve.

This modular configuration makes the system suitable for automated method development, column selection, condition scouting, sequential/tandem purification and complex Multi-D chromatographic workflows.

Technical specifications

Specification Bio-Rad NGC Quest 10 Plus

Type Automated preparative liquid chromatography system

Application Method development, protein/peptide/nucleic acid purification, Multi-D chromatography

Control Bio-Rad ChromLab Software

System pumps 2 × reciprocating piston pump, pump A + B

Pump configuration F10, up to 10 mL/min per pump

Adjustable flow rate F10 0.001–10 mL/min

Pressure range F10 0–25.2 MPa / 3,650 psi

Flow accuracy ±2% under specified conditions

Sample pump Piston/metering pump

Sample pump flow rate 0.01–100 mL/min

Sample pump accuracy ±2%

Sample pump pressure range 0–10 MPa / 1,450 psi

UV/Vis detector Multi-Wavelength Detector II

UV/Vis range 190–800 nm

Simultaneous wavelengths Max. 4

Absorbance range 0–3.0 AU

Linearity 0–2.5 AU within ±5%

Conductivity 0.01–999.9 mS/cm

Conductivity accuracy ±2%

Temperature measurement 4–60 °C

Inlet valves Inlet A: 8; Inlet B: 8; sample inlet: 8 or 15

Column switching Up to 3 × 5-port column switching valves within the NGC platform

Inject valve 1

Pressure sensor 0–3,650 psi; ±2 psi or 2%

Power supply 100–240 V AC, 50–60 Hz

Max. power 750 W

Operating temperature 4–40 °C

The official Bio-Rad specifications indicate a system size of approximately 49 × 61 × 56 cm (W × D × H) for the NGC Quest Plus systems and a weight of approximately 41–46 kg, depending on the version.

Multi-D configuration

The described version is more extensive than a standard NGC Quest 10 Plus. The combination of:

two F10 system pumps for A/B gradients;

multi-wavelength UV/Vis + conductivity detection;

separate 100 mL/min sample pump;

sample/inlet valve module;

column switching valve(s);

automated valve control;

ChromLab software;

support Bio-Rad Multi-D plumbing and methods,

makes the system suitable for automated multi-dimensional chromatography. Bio-Rad has described a specific Multi-D configuration for this purpose, in which an NGC Quest 10 Plus is equipped with, among other things, a sample pump, buffer inlet valves, two column switching valves and an outlet valve.