Agilent 1200/6410 Triple Quad LC/MS System

System consists of:
Agilent 1200 Solvent Tray
Agilent 1200 G1379B Degasser
Agilent 1200 G1312B Binary Pump SL
Agilent 1200 G1367B High Performance Autosampler
Agilent 1200 G1316A Thermostatted Column Compartment
Agilent 6410 Triple Quadrupole LC/MS
Edwards E2M30 vacuum pump
PC

G1379B Degasser:
Maximum flow rate: 0 – 5 ml/min per channel
Number of channels: 4
Internal volume per channel: Typically 1 ml per channel
Materials in contact with solvent: PTFE, FEP,PEEK
pH range: 1 – 14

G1312B Binary Pump SL:
Setable flow range: Set points 0.001 – 5 mL/min, in 0.001 mL/min increments
Flow range: 0.05 – 5.0 mL/min
Flow precision: ≤0.07 % RSD or ≤0.02 min SD, whatever is greater
Flow accuracy: ± 1 % or 10 µL/min, what ever is greater
Pressure operating range: Operating range 0 – 60 MPa (0 – 600 bar, 0 – 8700 psi) up to 5 mL/min
Pressure pulsation: < 2 % amplitude (typically < 1.3 %), or < 0.3 MPa (3 bar), whatever is greater, at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar, 147 psi)
Recommended pH range: 1.0 – 12.5 , solvents with pH < 2.3 should not contain acids which attack stainless
steel
Gradient formation: High-pressure binary mixing
Delay volume: Standard delay volume configuration:
600 – 800 µL, (includes 400 µL mixer), dependent on back pressure
Composition range settable range: 0 – 100 %, recommended range: 1 – 99 % or 5 µL/min per channel, whatever is greater
Composition precision: < 0.15 % RSD or < 0.04 min SD whatever is greater
Composition accuracy: ± 0.35 % absolute, at 2 mL/min, at 10 MPa (100 bar)

G1367B High Performance Autosampler:
Injection range: 0.1 – 100 µl in 0.1 µl increments
Precision Typically: < 0.25% RSD from 5 – 100 µl, Typically < 1% RSD from 1 – 5 µl variable volume
Pressure range: 400 bar (5880 psi)
Sample viscosity range: 0.2 – 5 cp
Injection cycle time: Typically < 30 s using following standard conditions
Carry-over: Typically < 0.01%

G1316A Thermostatted Column Compartment:
Temperature range: 10 degrees below ambient to 80 °C (flow rates up to 5 ml/min)
Temperature stability: ± 0.15 °C
Temperature accuracy: ± 0.8 °C
Column capacity: Three 30 cm
Warm-up/cool-down time: 5 minutes from ambient to 40 °C, 10 minutes from 40 – 20 °C
Dead volume: 3 µl left heat exchanger, 6 µl right heat exchanger

G6410A Triple Quadrupole LC/MS
MS/MS sensitivity and repeatability: Signal-to-noise ratio >20:1, where noise = 5x RMS
Mass resolution: Three settings (FWHM), Unit: 0.7 u; Wide: 1.2 u; Widest: 2.5 u
Mass accuracy: 0.1 u across the mass range
Mass stability: <0.1 u in 24 h
Dynamic range: >6.5 x 10⁶
Mass range:15 – 1,650 m/z
Scan rate: 5,200 u/sec
Scan modes: MS scan, MS/MS product ion scan, MRM, MS/MS neutral loss/gain scan and precursor ion scan, SIM
Minimum MRM dwell: time 5 ms with 10 MRMs
MRM transitions: 99 per time segment, thousands possible with multiple segments
Positive/negative polarity switching: Inter-segment delay of 1.5 sec
Collision cell: High-pressure, hexapole with linear acceleration
Cross-talk: None detectable
Single point of control: Single-point data system method capability with full control of Agilent 1100 and 1200 Series HPLC systems and 6410 Triple Quadrupole LC/MS
Time programming:
• Positive/negative polarity switching in a time segment
• Scan and SIM or MRM (plus other modes of data collection)
• Solvent divert through calibrant delivery system valve
• Possible 99-time segments with potential 99 MRMs per-time window
Autotune: Automated optimization of ion optics and mass axis calibration
Solvent declustering: Countercurrent gas
Detector: High-energy conversion dynode and removable electron multiplier horn
Dimensions (HxWxD): 470 x 1110 x 660 mm
Weight: 108 kg