GE Healthcare Cytiva Xuri W25 Cell Expansion Bioreactor System

The Xuri Cell Expansion System W25 delivers an automated, controlled expansion environment for the growth of cell therapies in volumes up to 25 L across research, process development, and manufacturing operations.

Closed system minimizes risk of cell contamination.

High cell densities through automated perfusion protocols.

Processes stay in control through advanced control capabilities and remote operation/monitoring via UNICORN software.

Suitable for use in a regulated environment.

Achieve >10 x 10⁶ cells/mL, in volumes up to 25 L, with non-invasive mixing, media perfusion, and efficient oxygen transfer in a low-sheer environment.

External air supply (per CBCU): 1.0 to 1.5 bar, Normal use: 1.3 L/min, Fast fill: 3.5 L/min

External CO2 supply (per CBCU): 1.0 to 1.5 bar, Normal use: 0.2 L/min, Fast fill: 0.5 L/min

External O2 supply (per CBCU): 1.0 to 1.5 bar, Normal use: 0.7 L/min, Fast fill: 1.7 L/min

Rocker specifications

Rocking speed control range: 2 to 40 rpm

Rocking angle control range: 2° to 12°

Rocking speed profile range: 15% to 100% (The % value refers to the fraction of the angular displacement function that is sinusoidally shaped 15% gives an almost constant angular velocity 100% gives a sinusoidal rocking)

Media weight control range: 0.5 to 25 kg

Scale, absolute accuracy (single mode): ±(0.050 + 1% of load) kg

Scale, left/right absolute accuracy (dual mode): ±(0.1 + 6% of load) kg

Temperature sensor: Pt100 Class A

Temperature measurement range: 2°C to 50°C

Temperature setpoint difference (dual mode): Max 10°C at ambient 21°C (The setpoint difference is reduced by 1°C for every degree centigrade that is increased from ambient temperature (e.g., at ambient 25°C, max setpoint difference is 6°C))

Temperature control range: (Ambient temperature + 5°C) to 40°C

Temperature control accuracy (excl. measurement error): ±0.2°C

CBCU specifications

Gas flow control range: 50 to 1000 mL/min

Total gas flow accuracy (reference flow – setpoint): ±(10 + 3% of read value) mL/min

Fast fill flow: ~3 L/min

CO2 control range: 0% to 15% CO2

CO2 measurement accuracy at 5% CO2: ±0.5% CO2 when mixed only with air/N2

CO2 control accuracy (versus setpoint): ±0.4% CO2

O2 control range: 0% to 50% O2 when mixed with N2, 21% to 50% O2 when mixed with air

O2 measurement accuracy: ±(0.6 + 1% of read value) within 0% to 50% O2, when mixed only with air/N2

O2 control accuracy (versus setpoint): ±0.6% O2

pH measurement range: pH 4.5 to 8.5

pH control range: pH 6.0 to 8.0

pH measurement accuracy: ±0.05 pH within ±0.25 pH from offset calibration pH, ±0.1 pH within 0.25 to 0.5 pH from offset calibration pH

pH control accuracy (versus setpoint): ±0.05 pH

DO measurement range: 0% to 250% air saturation

DO measurement accuracy: ±5% air saturation, excluding atmospheric pressure variations

DO control range: 0% to 100% air saturation

Pump specifications

Pump flow rate range: 0.1 to 144 L /day (0.07 to 100 mL/min)

Pump flow rate accuracy: ±(0.1 + 5% of read value) mL/min after calibration

Accumulated pumped volume accuracy: ±10% of measured volume

Supported tubing dimensions: Inner diameter 0.5 to 4.8 mm (1/50″ to 3/16″), Wall thickness 1.6 mm (1/16″)

Dimensions rocker (WxHxD): 404 x 205 x 560 mm

Dimensions CBCU (WxHxD): 276 x 117 x 360 mm

Dimensions pump (WxHxD): 275 x 115 x 280 mm

Weight rocker: 24 kg

Weight CBCU: 4.8 kg

Weight pump: 3.8 kg

Excluding software