Illumina MiSeq Sequencing System

The MiSeq System offers the first DNA-to-data sequencing platform integrating cluster generation, amplification, sequencing, and data analysis into a single instrument. Its small footprint—approximately two square feet—fits easily into virtually any laboratory environment. The MiSeq System leverages Illumina sequencing by synthesis (SBS) chemistry, a proven next-generation sequencing (NGS) technology cited in over 300,000 peer-reviewed publications—5× more than all other NGS technologies combined. With the power of NGS delivered in a compact footprint, the MiSeq System is the ideal platform for rapid and cost-effective genetic analysis.

MiSeq System performance parameters:

MiSeq Reagent Kit v2

Read length

2 × 25 bp: Total time – ~5.5 hours, Output – 750-850 Mb, Quality scores – > 90% bases higher than Q30, Single reads – 12-15M, Paired-end reads – 24-30M

2 × 150 bp: Total time – ~24 hours, Output – 4.5-5.1 Gb, Quality scores – > 80% bases higher than Q30, Single reads – 12-15M, Paired-end reads – 24-30M

2 × 250 bp: Total time – ~39 hours, Output – 7.5-8.5 Gb, Quality scores – > 75% bases higher than Q30, Single reads – 12-15M, Paired-end reads – 24-30M

MiSeq Reagent Kit v3

Read length

2 × 75 bp: Total time – ~21 hours, Output – 3.3-3.8 Gb, Quality scores – > 85% bases higher than Q30, Single reads – 22-25M, Paired-end reads – 40-50M

2 × 300 bp: Total time – ~56 hours, Output – 13.2-15 Gb, Quality scores – > 70% bases higher than Q30, Single reads – 22-25M, Paired-end reads – 40-50M

MiSeq Reagent Kit v2 Micro

2 × 150 bp: Total time – ~19 hours, Output – 1.2 Gb, Single reads – 4M, Paired-end reads – 8M

MiSeq Reagent Kit v2 Nano

2 × 150 bp: Total time – ~17 hours, Output – 300 Mb, Single reads – 1M, Paired-end reads – 2M

2 × 250 bp: Total time – ~28 hours, Output – 500 Mb, Single reads – 1M, Paired-end reads – 2M

Light emitting diode (LED): 520 nm, 660 nm

Dimensions (HxWxD): 523 x 686 x 565 mm

Weight: 57,2 kg