Molecular Devices SpectraMax i3x Microplate Reader

The SpectraMax i3x Multi-Mode Microplate Reader can perform a wide range of essential cell-based and biochemical assays using the included absorbance, fluorescence, and luminescence detection modes.

Read modes: Absorbance, Fluorescence (top/bottom read), Luminescence (top read), Time-Resolved Fluorescence (top read), Fluorescence Polarization (top read), HTRF (top read), AlphaScreen (top read)

Wavelength ranges: Abs: 230 – 1000 nm, FL Ex: 250 – 830 nm, FL Em: 270 – 850 nm, Lumi: 300 – 850 nm

Wavelength selection: Cartridge filters and monochromator tunable in 1 nm increments

Absorbance photometric accuracy/linearity: < ±0.010 OD ±1.0%, 0 – 3 OD VIS 0 – 2 OD UV

Absorbance photometric precision/repeatability: < ±0.003 OD ±1.0%, 0 – 3 OD VIS 0 – 2 OD UV

Sensitivity top read optimized: FL: Mono: 0.5 pM fluorescein 96 well, 1 pM fluorescein 384 well; Cartridge: 0.1 fmol/well in 75 μL fluorescein 384 well, 0.03 fmol/well in 8 μL fluorescein 1536 well

Lumi: Mono: 3 pM ATP-Glow 96 well, 6 pM ATP-Glow 384 well; Cartridge: 20 amol ATP-flash

TRF cartridge: 3 amol/well in 100 μL europium (0.03 pM) 384 well, 1 amol/well in 8 μL europium (0.125 pM) 1535 well

FP cartridge: 3 mP (75 μl) fluorescein 384 well, 6 mP (8μl) fluorescein 1536 well

AlphaScreen cartridge: <100 amol/well 384 well

Light source(s): High power xenon flash lamp, LEDs in visible range

Detector(s): Silicon photodiode, Cooled photomultiplier tube

Read types: Endpoint, Kinetic, Spectrum scan, Well scan

Read times: Abs: 29 seconds, FL: 22 seconds, Lumi: 21 seconds, TRF: 21 seconds, FP: 38 seconds, AS: 22 seconds

Plate shaking: Linear, Orbital

Plate type(s): 6-1536 well plates

Temperature control: Ambient + 4°C to 45°C

Dimensions (HxWxD): 325 x 392 x 605 mm

Weight: 31 kg