Illumina NextSeq 500 Sequencing System

The NextSeq 500 system whole-genome sequencing (WGS) solution enables researchers and clinicians to explore the entire genome of any species cost effectively for a deeper understanding of biology. It leverages industry-standard Illumina next-generation sequencing (NGS) technology responsible for most global WGS, delivering the best data quality and highest coverage to identify variants in coding and noncoding regions of the genome. High-quality ;ibrary preperation kits are optimized for low-imput, unbiased coverage, and rapid workflow. With push-button sequencing, simple data analysis, and minimal hands-on time, the NextSeq 500 system WGS solution enables researchers to interrogate simple prokaryotic and complex eukaryotic genomes quickly and efficiently.

NextSeq 500 performance parameters:

High output flow cell
Read length:
2 × 150 bp: Total time – ~29 hours, Output – 100-120 Gb, Single reads – 400M, Paired-end reads – 800M, Required input – 100 ng-1 µg with TruSeq Library Prep Kits, Data quality – >75% higher than Q30 at 2 × 150 bp
2 × 75 bp: Total time – ~18 hours, Output – 50-60 Gb, Single reads – 400M, Paired-end reads – 800M, Required input – 100 ng-1 µg with TruSeq Library Prep Kits, Data quality – >75% higher than Q30 at 2 × 150 bp
1 × 75 bp: Total time – ~11 hours, Output – 25-30 Gb, Single reads – 400M, Paired-end reads – 800M, Required input – 100 ng-1 µg with TruSeq Library Prep Kits, Data quality – >75% higher than Q30 at 2 × 150 bp

Mid output flow cell
Read length:
2 × 150 bp: Total time – ~26 hours, Output – 32-39 Gb, Single reads – 130M, Paired-end reads – 260M, Required input – 100 ng-1 µg with TruSeq Library Prep Kits, Data quality – >75% higher than Q30 at 2 × 150 bp
2 × 75 bp: Total time – ~15 hours, Output – 16-19 Gb, Single reads – 130M, Paired-end reads – 260M, Required input – 100 ng-1 µg with TruSeq Library Prep Kits, Data quality – >75% higher than Q30 at 2 × 150 bp

Light emitting diode (LED): 520 nm, 650 nm; Laser diode 780 nm
Dimensions (HxWxD): 635 x 585 x 534 mm
Weight: 83 kg