Amersham Biosciences Ettan IPGphor II Isoelectric Focusing System

The Ettan IPGphor Isoelectric Focusing System performs first dimension isoelectric focusing (IEF) in 2-dimensional (2-D) electrophoresis.

The system comprises Immobiline DryStrip gel strips, which contain an immobilized pH gradient (IPG); strip holders, which serve as both rehydration and IEF chambers for the IPG strips; and the Ettan IPGphor unit, which includes a high-voltage DC power supply, solid state temperature control, and can be programmed with up to ten user-defined IEF protocols. Programmable functions include:

• rehydration time

• platform temperature

• maximum current limit

• voltage change pattern

• voltage for each step in the isoelectric focusing program, and  step duration.

Working surface

Electrode areas: Gold-plated copper

Strip holder capacity: Up to 12 fixed-length, up to 9 cup loading

Platform temperature: 18°C – 25°C, ±1°C

Strip holders

Material: Aluminum oxide ceramic base, acrylic cover, polycarbonate, platinum on titanium

Holders for IPG strip lengths: 70, 110, 130, 180, and 240 mm

User interface

Control panel: 7-key membrane keypad.

Liquid crystal display (LCD): 2 lines, 16 characters per line

Programmable parameters: Rehydration time, platform temperature, maximum current limit per strip, step voltage, step voltage change pattern, and step duration.

Protocol capacity: 10, with up to 9 steps each

Power outputs

Voltage: 0 – 8000 V, resolution 10 V

Current 0 – 1.5 mA, resolution 0.01 mA

Power:  12 W max

Physical parameters

Dimensions (HxWxD): 140 x 250 x 460 mm

Weight: 6.8 kg