Bio-Rad Gene Pulser II Electroporation System

The Gene Pulser II system is a completely electroporation system, whichoffers improved performance, flexibility, sample protection, and safety. The Gene Pulser II system uses the Pulse Trac waveform delivery system to generate the most accurate expo-nential decay pulses possible for optimal cell transformation within an electroporation cuvette.The Pulse Trac delivery system accurately calculates the time constant of each pulse based on

what is actually delivered to the sample.

The revolutionary Pulse Trac waveform delivery system provides:

Sample conductivity measurement integrated with pulse output for true waveform deliveryregardless of sample

Internal calibration and circuit monitoring program for accurate pulse delivery throughout thelifetime of the unit

Improved time constant accuracy

Error free assembly, since each module uses unique cable connectors, keyed to fit only in theproper orientation and location

Automatic display of time constant after every pulse, to make it easier to record this essen-tial pulse parameter

Easier optimization of novel prokaryotic applications with a wider range of high voltagecapacitors (1.0 , 3.0, 10, 25, and 50 μF) in the Gene Pulser II unit

Pinpointing of optimal field strengths, with voltage selection in 2 V increments for mam-malian cell electroporation (low voltage/high capacitance) and operation at a higher maximumvoltage (up to 500 V). In addition, voltage selection in 10 V increments for bacterial (high volt-age/low capacitance) electroporation

Increased control of high voltage pulses used for bacteria and yeast electro-transformation withthe five new resistors in the Pulse Controller PLUS module

Designed and certified to meet IEC 1010 safety standards

Entire system is completely protected from sample arcs, regardless of module or sample used

Expanded safety, with charges automatically diverted safely to ground whenever the circuitis interrupted during pulse delivery.

The Gene Pulser II unit comes with a Pulse Controller PLUS module for electroporation of bacteria and yeast.

Gene Pulser II System:

Maximum output voltage and current: 2,500 V at 125 amp peak (normal load); limited to 1,500 A during arc.

Output waveform: Pulse Trac exponential decay with RC time con-stant dependent on sample and capacitor selected

Output voltage adjustment: 50–2,500 V range (depending on the capacitor) with 10 V adjustment precision for the high voltage range and 2 V for the low-voltage range(50–500 V).

Regulatory: Passes requirements of IEC 1010. In addition, thesystem passes requirements for FCC, Class A.

Dimensions: 340 x 310 x 190 mm

Weight: 10.41 kg

Pulse Controller PLUS System:

Selectable resistance, ohms, (parallel): 50, 100, 200, 300, 400, 500, 600, 700, 800, 900,1,000, infinity ohms; measured with Gene Pulser II apparatus

Dimensions: 230 x 310 x 77 cm

Weight: 1.87 kg