Agilent 1260 Infinity Preparative HPLC System

Agilent 1260 Infinity system consisting of:

2x Agilent 1260 Prep Pump

1x Agilent 1260 FC-PS Preparative Scale Fraction Collector

1x Agilent 1260 Multiple Wavelength Detector

Solvent Tray

Manual injector with a 10 ml sample loop

PC and software

Agilent 1260 Prep Pump:

The Agilent 1200 Series preparative pump delivers a stable and reproducible flow at both low and high flow rates. By adding a second pump, a high-pressure gradient system can be assembled easily.

Features:

Flow rates up to 100 mL/min at 400 bar without changing pump heads

Precise high-pressure binary gradient formation with low delay volume for preparative and analytical applications with flow rates as low as 0.5 mL/min

Extended seal lifetime by automated seal wash

Setting upper and lower pressure limits will turn off the pump, e.g. if the column gets blocked or leaks occur

Convenient system setup by automated electromagnetic purge valve

Specifications:

Flow precision: <0.3 % RSD in range from 1 mL/min to 100 mL/min

Hydraulic system: Dual pistons in parallel

Flow range: Setpoints from 0.001 to 100 mL/min, in 0.001 mL/min increments. Recommended: 5 – 100 mL/min

Pressure: Operating range 2 – 40 MPa (20 – 400 bar, 290 – 5880 psi) system pressure

Gradient formation: High pressure using two combined pumps

Delay volume: 700 µL

pH range: 1.0 – 12.5 recommended

Agilent 1260 FC-PS Preparative Scale Fraction Collector

Specifications:

Ttrigger modes: Time slices, Peak (threshold, up- / downslope),

Timetable (combination of time intervals and peak).

Operating modes: Discrete fractions –  default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position.

Fraction capacities: Full tray with 126 test tubes (16 mm OD, max. height 100 mm, ~12 ml/tube)

Installed trays are automatically detected and identified.

For the with uncapped vials, tests tubes and well plates, only!

Maximum tube volume: ca. 45 ml

Maximum flow rate: 100 ml/min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve)

Delay volumes [μl]: Fraction collector inlet to diverter valve: ~500 (typical, depends on length of the tubing), Diverter valve: ~15, Diverter valve to needle: ~110, Needle: ~5

Delay calibration sensor: Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode

Diverter valve: 3/2 Diverter valve with low internal volume (15 μl), switching time < 100 ms, maximum operating pressure 6 bar

Agilent 1260 Multiple Wavelength Detector

Specifications:

Detection type: 1024-element photodiode array

Light source: Deuterium and tungsten lamps

Data rate: Up to 20 Hz

Wavelength range: 190 – 950 nm

Short term noise (ASTM) Single and Multi-wavelength: < ± 0.7·10AU at 254 and 750 nm

Drift: < 0.9·10AU/h at 254 nm

Linear absorbance range: > 2 AU (5 %) at 265 nm

Wavelength accuracy: ± 1 nm

Wavelength bunching: 1 – 400 nm

Slit width: 1, 2, 4 , 8, 16 nm

Diode width: < 1 nm

Flow cell: 13 µl volume, 10 mm cell length

Time programmable: Wavelength, polarity, peak width,lamp bandwidth, autobalance,wavelength range, threshold, spectrastorage mode